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Competitive Homing Assays to Study Gut-tropic T Cell Migration
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Competitive Homing Assays to Study Gut-tropic T Cell Migration

Competitive Homing Assays to Study Gut-tropic T Cell Migration

DOI:
10.3791/2619-v

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10:25 min

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March 01, 2011

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,
1Gastrointestinal Unit,Massachusetts General Hospital, Harvard Medical School

Capítulos

  • 00:05Título
  • 01:13Introduction
  • 01:59Ex vivo Generation of Gut-Homing and Control T cells
  • 04:25Analysis of Gut-Homing Receptors on Activated T cells
  • 05:21T cell Labeling with CFSE and CMTMR Cell Trackers
  • 07:42Analysis of T cell Homing
  • 08:58RA-induced Expression of a4B7 and CCR9 Direct T Cell Migration to the Gut
  • 09:50Conclusion

Summary

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Competitive homing experiments allow to directly assessing the migratory properties of two different cell populations in a single mouse. Here we illustrate this procedure by comparing the migration of ex vivo-generated gut-tropic versus non-gut tropic T cells.

Tags

Competitive Homing AssaysGut-tropic T Cell MigrationLymphocyte AdhesionAdhesion MoleculesHoming ReceptorsAddressinsEndothelial CellsIntravital Microscopy (IVM)Homing ExperimentsMigration AnalysisCell SubsetsMouse Model

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