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Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
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Immunology and Infection
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JoVE Journal Immunology and Infection
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

45,907 Views

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08:39 min

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June 01, 2010

DOI:

10.3791/2017-v

DOI:
10.3791/2017-v

•

08:39 min
June 01, 2010

•

5 Views
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1Center for Vaccine Biology and Immunology,University of Rochester

Tags

T Lymphocyte IsolationCultureMigration AnalysisIntegrinsLigandsExtracellular Matrix ProteinsCell Leading EdgeUropodLeukocyte RecruitmentAutoimmune DiseasesInflammatory DiseasesIn Vitro SystemTwo-dimensional SubstrateIntercellular Adhesion Molecule-1 (ICAM-1)Integrin LFA-1Stromal Cell-derived Factor-1 (SDF-1)Migratory VelocityIntegrin BlockadeActomyosin Machinery

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Lefort, C. T., Kim, M. Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro. J. Vis. Exp. (40), e2017, doi:10.3791/2017 (2010).

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