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Competitivo saggi Homing allo Studio Gut-tropico Migrazione cellulare T
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Competitive Homing Assays to Study Gut-tropic T Cell Migration
DOI:

10:25 min

March 01, 2011

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Chapters

  • 00:05Title
  • 01:13Introduction
  • 01:59Ex vivo Generation of Gut-Homing and Control T cells
  • 04:25Analysis of Gut-Homing Receptors on Activated T cells
  • 05:21T cell Labeling with CFSE and CMTMR Cell Trackers
  • 07:42Analysis of T cell Homing
  • 08:58RA-induced Expression of a4B7 and CCR9 Direct T Cell Migration to the Gut
  • 09:50Conclusion

Summary

Automatic Translation

Esperimenti di homing competitivo permettono di valutare direttamente le proprietà migratorie di due diverse popolazioni cellulari in un mouse. Qui illustrare questa procedura confrontando la migrazione di ex vivo generata gut-tropico rispetto a non-intestinali cellule T tropico.

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