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迁移人T淋巴细胞分离，培养和分析<em在体外</em
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Immunology and Infection
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JoVE Journal Immunology and Infection
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
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迁移人T淋巴细胞分离，培养和分析<em在体外</em

DOI:
10.3791/2017-v

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08:39 min

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June 01, 2010

•

,
1Center for Vaccine Biology and Immunology,University of Rochester

Chapters

  • 00:00Title
  • 00:37Introduction
  • 01:31Isolation of Human T Lymphocytes
  • 02:57Culture of Human T Lymphocytes
  • 05:04In Vitro Lymphocyte Migration Assay
  • 06:15Capturing an Image Sequence Using NIS Elements Software
  • 07:20Representative Results
  • 08:12Conclusion

Summary

Automatic Translation

Automatic Translation

T淋巴细胞的迁移过程中出现归巢淋巴器官，从血管中退出，进入周围组织。在这里，我们描述了一个可以用来分析T淋巴细胞迁移的协议<em>在体外</em>。

Tags

T Lymphocyte IsolationCultureMigration AnalysisIntegrinsLigandsExtracellular Matrix ProteinsCell Leading EdgeUropodLeukocyte RecruitmentAutoimmune DiseasesInflammatory DiseasesIn Vitro SystemTwo-dimensional SubstrateIntercellular Adhesion Molecule-1 (ICAM-1)Integrin LFA-1Stromal Cell-derived Factor-1 (SDF-1)Migratory VelocityIntegrin BlockadeActomyosin Machinery

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