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Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro
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Immunology and Infection
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JoVE Journal Immunology and Infection
Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro

Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro

DOI:
10.3791/58759-v

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08:31 min

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November 26, 2018

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, , , ,
1Institute of Biochemistry and Cell Biology,OVGU University of Magdeburg

Chapters

  • 00:04Title
  • 00:43Preparing the 6-chambered Slides for the Flow Experiment
  • 01:49Fluidics Unit Preparation
  • 03:07Cell Loading
  • 04:04Attaching the Fluidics Unit and Imaging the Cells
  • 05:28Migration Track Analysis
  • 07:02Results: Quantifying Marginal Zone B-cell Shuttling
  • 08:00Conclusion

Summary

Automatic Translation

Automatic Translation

Marginal zone B cells (MZBs) respond to the force of shear flow by re-orienting their migration path up the flow. This protocol shows how to record and analyze the migration using a fluidics unit, pump, microscope imaging system, and free software.

Tags

Shear FlowMarginal Zone B CellsMigration AssayICAM-1LeukocytesCytoskeletonIntegrin LigandsChemokinesActivated T CellsFluid PumpMigration BufferShear Stress

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