Journal
/
Immunology and Infection
/
Isolamento T de linfócitos humanos, Cultura e Análise da Migração In Vitro</em
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

Isolamento T de linfócitos humanos, Cultura e Análise da Migração In Vitro</em

português

Automatically Generated

45,971 Views

•

08:39 min

•

June 01, 2010

DOI:

10.3791/2017-v

DOI:
10.3791/2017-v

•

08:39 min
June 01, 2010

•

16 Views
,
1Center for Vaccine Biology and Immunology,University of Rochester

Summary

Automatically generated

Migração de linfócitos T ocorre durante homing para órgãos linfóides, saia do vasculatura, e entrando em tecidos periféricos. Aqui, nós descrevemos um protocolo que pode ser usado para analisar migração de linfócitos T<em> In vitro</em>.

Explore More Videos

T Lymphocyte IsolationCultureMigration AnalysisIntegrinsLigandsExtracellular Matrix ProteinsCell Leading EdgeUropodLeukocyte RecruitmentAutoimmune DiseasesInflammatory DiseasesIn Vitro SystemTwo-dimensional SubstrateIntercellular Adhesion Molecule-1 (ICAM-1)Integrin LFA-1Stromal Cell-derived Factor-1 (SDF-1)Migratory VelocityIntegrin BlockadeActomyosin Machinery

Read Article

Cite this Article

Lefort, C. T., Kim, M. Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro. J. Vis. Exp. (40), e2017, doi:10.3791/2017 (2010).

Download .ris file

Copy

Share Video

.

Copy