Efficient Transfection of <em>In vitro</em> Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Qin Xiao; Yuheng Liu; Dandan Zhang; Chao Li; Qihua Yang; Dongshui Lu; Weijun Zhang; Joseph Rosenecker; Quanming Zou; Yang Li; Shan Guan

doi:10.3791/64288

/

Efficiënte transfectie van in vitro getranscribeerd mRNA in gekweekte cellen met behulp van peptide-poloxamine nanodeeltjes

JoVE Journal
İmmünoloji ve Enfeksiyon

Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.

JoVE Journal İmmünoloji ve Enfeksiyon

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Please note that all translations are automatically generated. Click here for the English version.

Efficiënte transfectie van in vitro getranscribeerd mRNA in gekweekte cellen met behulp van peptide-poloxamine nanodeeltjes

DOI:

10.3791/64288-v

•

10:16 min

•

August 17, 2022

•

Qin Xiao*¹, Yuheng Liu*¹, Dandan Zhang, Chao Li, Qihua Yang, Dongshui Lu, Weijun Zhang, Joseph Rosenecker, Quanming Zou, Yang Li, Shan Guan

¹National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy,Third Military Medical University, ²Pediatri Anabilim Dalı,Ludwig-Maximilians University of Munich, ³Department of Pharmacy, Southwest Hospital,Third Military Medical University

Bölümler

00:05Introduction
01:19In Vitro Transcription (IVT) of Chemically Modified mRNA
02:55Generation of IVT mRNA/PP-sNps
05:22Preparation of the Cells for Transfection and Transfection of the Cultured Cells
07:04Analysis of Cell Transfection Efficacy Using Metridia Luciferase (MetLuc) Assay
07:53Results: Analyzing the Transfection Efficiency of MetLuc-mRNA/PP-sNp
09:12Conclusion

Özet

Otomatik Çeviri

Een zelfgeassembleerd peptide-poloxamine nanodeeltje (PP-sNp) wordt ontwikkeld met behulp van een microfluïdisch mengapparaat om in vitro getranscribeerd boodschapper-RNA in te kapselen en af te leveren. Het beschreven mRNA/PP-sNp kon in vitro efficiënt gekweekte cellen transfecteren.

Etiketler

MRNA Transfection Peptide-poloxamine Nanoparticles Mucosal MRNA Vaccine Lung Cancer Cystic Fibrosis In Vitro Transcription Capped MRNA Agarose Gel Electrophoresis Poloxamine 704 Synthetic Peptide

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Retrovirale Transductie van T-cel-receptoren in Mouse T-cellen

Genotypische Inferentie van HIV-1 tropisme de populatie-gebaseerde Sequencing van V3

Zeven Stappen naar cellen stellatum

Geoptimaliseerd protocol voor Efficiënte Transfectie van dendritische cellen zonder Cell Rijping

Kwantitatief<em> In vitro</em> Assay om neutrofieladhesie Maatregel om Activated primaire humane microvasculaire endotheelcellen onder statische omstandigheden