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Effiziente Transfektion von in vitro transkribierter mRNA in kultivierten Zellen mit Peptid-Poloxamin-Nanopartikeln
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JoVE Journal İmmünoloji ve Enfeksiyon
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
Please note that all translations are automatically generated. Click here for the English version.

Effiziente Transfektion von in vitro transkribierter mRNA in kultivierten Zellen mit Peptid-Poloxamin-Nanopartikeln

2,886 Views

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10:16 min

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August 17, 2022

DOI:

10.3791/64288-v

DOI:
10.3791/64288-v

•

10:16 min
August 17, 2022

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8 Views
, , , , , , , , , ,
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy,Third Military Medical University, 2Pediatri Anabilim Dalı,Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital,Third Military Medical University

Özet

Automatically generated

Ein selbstorganisiertes Peptid-Poloxamin-Nanopartikel (PP-sNp) wird unter Verwendung einer mikrofluidischen Mischvorrichtung entwickelt, um in vitro transkribierte Boten-RNA zu verkapseln und zu liefern. Die beschriebene mRNA/PP-sNp konnte kultivierte Zellen in vitro effizient transfizieren.

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Cite this Article

Xiao, Q., Liu, Y., Zhang, D., Li, C., Yang, Q., Lu, D., Zhang, W., Rosenecker, J., Zou, Q., Li, Y., Guan, S. Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles. J. Vis. Exp. (186), e64288, doi:10.3791/64288 (2022).

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