Dergi
/
İmmünoloji ve Enfeksiyon
/
使用肽-泊洛沙明纳米颗粒在培养细胞中高效转染 体外 转录的mRNA
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
Please note that all translations are automatically generated. Click here for the English version.

使用肽-泊洛沙明纳米颗粒在培养细胞中高效转染 体外 转录的mRNA

DOI:
10.3791/64288-v

•

10:16 min

•

August 17, 2022

•

, , , , , , , , , ,
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy,Third Military Medical University, 2Pediatri Anabilim Dalı,Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital,Third Military Medical University

Bölümler

  • 00:05Introduction
  • 01:19In Vitro Transcription (IVT) of Chemically Modified mRNA
  • 02:55Generation of IVT mRNA/PP-sNps
  • 05:22Preparation of the Cells for Transfection and Transfection of the Cultured Cells
  • 07:04Analysis of Cell Transfection Efficacy Using Metridia Luciferase (MetLuc) Assay
  • 07:53Results: Analyzing the Transfection Efficiency of MetLuc-mRNA/PP-sNp
  • 09:12Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

使用微流体混合装置开发自组装肽 - 泊洛沙明纳米颗粒（PP-sNp）以封装和递送 体外 转录的信使RNA。所描述的mRNA / PP-sNp可以在 体外有效地转染培养的细胞。

Etiketler

MRNA TransfectionPeptide-poloxamine NanoparticlesMucosal MRNA VaccineLung CancerCystic FibrosisIn Vitro TranscriptionCapped MRNAAgarose Gel ElectrophoresisPoloxamine 704Synthetic Peptide

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

T细胞受体在小鼠T细胞的逆转录病毒转导

V3的HIV - 1取向的基因型推理使用人口为基础的测序

星状细胞的七个步骤

高效转染树突状细胞的无细胞的成熟优化的协议

量<em在体外</em>激活的主人微血管内皮细胞在静态条件下测量的检测，以中性粒细胞粘附

利用荧光蛋白可视化和定量<em>衣原体</em>液泡生长动态的活细胞

杀手人工抗原提呈细胞（KaAPC）为有效<em>在体外</em>人抗原特异性T细胞耗竭

人THP-1巨噬细胞通过核转高效转染

对T细胞推导<em>在体外</em>从小鼠胚胎干细胞

一种简单有效的方法构建突变牛痘病毒载体

Read Article