Dergi
/
İmmünoloji ve Enfeksiyon
/
Trasfezione efficiente di mRNA trascritto in vitro in cellule videodan coltura utilizzando nanoparticelle peptide-poloxamina
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
Please note that all translations are automatically generated. Click here for the English version.

Trasfezione efficiente di mRNA trascritto in vitro in cellule videodan coltura utilizzando nanoparticelle peptide-poloxamina

2,886 Views

•

10:16 min

•

August 17, 2022

DOI:

10.3791/64288-v

DOI:
10.3791/64288-v

•

10:16 min
August 17, 2022

•

8 Views
, , , , , , , , , ,
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy,Third Military Medical University, 2Pediatri Anabilim Dalı,Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital,Third Military Medical University

Özet

Automatically generated

Una nanoparticella peptide-poloxamina autoassemblata (PP-sNp) viene sviluppata utilizzando un dispositivo di miscelazione microfluidica per incapsulare e fornire RNA messaggero trascritto in vitro . L'mRNA/PP-sNp descritto potrebbe trasfettare efficacemente le cellule in coltura in vitro.

Explore More Videos

MRNA TransfectionPeptide-poloxamine NanoparticlesMucosal MRNA VaccineLung CancerCystic FibrosisIn Vitro TranscriptionCapped MRNAAgarose Gel ElectrophoresisPoloxamine 704Synthetic Peptide

Read Article

Cite this Article

Xiao, Q., Liu, Y., Zhang, D., Li, C., Yang, Q., Lu, D., Zhang, W., Rosenecker, J., Zou, Q., Li, Y., Guan, S. Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles. J. Vis. Exp. (186), e64288, doi:10.3791/64288 (2022).

Download .ris file

Copy

Share Video

.

Copy