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Transfection efficace d’ARNm transcrit in vitro dans des cellules cultivées à l’aide de nanoparticules de peptide-poloxamine
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JoVE Journal İmmünoloji ve Enfeksiyon
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
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Transfection efficace d’ARNm transcrit in vitro dans des cellules cultivées à l’aide de nanoparticules de peptide-poloxamine

2,886 Views

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10:16 min

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August 17, 2022

DOI:

10.3791/64288-v

DOI:
10.3791/64288-v

•

10:16 min
August 17, 2022

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8 Views
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1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy,Third Military Medical University, 2Pediatri Anabilim Dalı,Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital,Third Military Medical University

Özet

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Une nanoparticule peptide-poloxamine auto-assemblée (PP-sNp) est développée à l’aide d’un dispositif de mélange microfluidique pour encapsuler et délivrer in vitro de l’ARN messager transcrit. L’ARNm/PP-sNp décrit pourrait transfecter efficacement des cellules cultivées in vitro.

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Cite this Article

Xiao, Q., Liu, Y., Zhang, D., Li, C., Yang, Q., Lu, D., Zhang, W., Rosenecker, J., Zou, Q., Li, Y., Guan, S. Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles. J. Vis. Exp. (186), e64288, doi:10.3791/64288 (2022).

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