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Journal / Biochemistry / 通过肽浓缩和质谱检测蛋白质泛化位点检测…
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JoVE Journal Biochemistry
通过肽浓缩和质谱检测蛋白质泛化位点检测
 
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通过肽浓缩和质谱检测蛋白质泛化位点检测

Article DOI: 10.3791/59079-v • 11:54 min • March 23rd, 2020
March 23rd, 2020 •
1, 1, 1
1Proteomics Center, Erasmus University Medical Center

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我们提出了一种从复杂生物样本中泛化的蛋白质中产生的二Gly肽的纯化、检测和鉴定方法。在泛源分析的深度级别方面，该方法具有可重复性、健壮性，优于已发布的方法。

Tags

生物化学， 第157期， 泛化， 泛素， 泛素， 翻译后修饰 （PTM）， 二Gly肽， 免疫纯化， 轨道拉质谱
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