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氢-铀交换质谱仪(HDX-MS)平台,用于研究肽生物合成酶
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
A Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Platform for Investigating Peptide Biosynthetic Enzymes
DOI:

11:32 min

May 04, 2020

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Capítulos

  • 00:04Introduction
  • 00:36Bottom-Up Liquid Chromatography-Mass Spectrometry (LC-MS) Reference Sample Workflow Preparation
  • 01:18Reference Data Processing and Peptide List Definition
  • 05:05Hydrogen-Deuterium Exchange (HDX) Reactions
  • 06:36HDX Data Processing, Analysis, and Visualization
  • 09:03Results: Reapresentative HDX-MS Peptide Biosynthetic Enzyme Evaluation
  • 10:44Conclusion

Summary

Tadução automática

在肽天然产品的生物合成过程中,兰西肽合成器催化多步反应。在这里,我们描述了一个连续的,自下而上的,氢-钚交换质谱仪(HDX-MS)的工作流程,可用于研究兰西肽合成器的构象动力学,以及肽天然产品生物合成中涉及的其他类似酶。

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