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肽聚糖的液相色谱质谱和生物信息学半定量分析

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

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肽聚糖的液相色谱质谱和生物信息学半定量分析

DOI:

10.3791/61799-v

•

09:09 min

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October 13, 2020

•

Erin M. Anderson, Neil A. Greenwood, Dyanne Brewer, Cezar M. Khursigara

¹Department of Molecular and Cellular Biology,University of Guelph, ²Mass Spectrometry Facility,University of Guelph

Capítulos

00:05Introduction
01:07Peptidoglycan Sacculi Extraction
03:17Liquid Chromatography-Mass Spectrometry (LC-MS) Chromatogram Spectral Processing
04:11Differential Spectral Feature Analysis
05:06Muropeptide Annotation Spectral Features
06:18Muropeptide Global Change Modification Assessment
07:07Results: Representative Muropeptide Fragmentation and Identification and Peptidoglycan Composition Analysis
08:31Conclusion

Summary

Tadução automática

该协议涵盖了使用液相色谱质谱结合高级特征提取和生物信息学分析软件对肽聚糖组成的详细分析。

Tags

Keywords: Peptidoglycan Mass Spectrometry Bioinformatics Peptidoglycomics Sacculi Extraction SDS Treatment Enzymatic Digestion Liquid Chromatography-mass Spectrometry

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