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使用液体色谱加上串联质谱法的糖核酸菌的定量元细胞学

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

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使用液体色谱加上串联质谱法的糖核酸菌的定量元细胞学

DOI:

10.3791/62061-v

•

07:25 min

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January 05, 2021

•

Karamat Mohammad, Heng Jiang, Vladimir I. Titorenko

¹Department of Biology,Concordia University, ²Centre for Biological Applications of Mass Spectrometry,Concordia University

Capítulos

00:04Introduction
00:42Cell Transfer and Culture
01:21Metabolite Extraction
02:44Extracted Metabolite Separation and Analysis
04:04Membrane Integrity Assessment
05:24Results: Representative Metabolic Intermediate and Product Identification and Quantification
06:50Conclusion

Summary

Tadução automática

我们提出了一个协议，用于识别和量化主要类别的水溶性代谢物在酵母糖体中。所述方法通用、坚固且灵敏。它允许结构异构体和立体形式的水溶性代谢物彼此分离。

Tags

Keywords: Quantitative Metabolomics Saccharomyces Cerevisiae Liquid Chromatography Tandem Mass Spectrometry Quenching Metabolite Extraction Chloroform Methanol Glass Beads Vortex Aqueous Phase Acetonitrile Liquid Chromatography-tandem Mass Spectrometry

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