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Immunology and Infection

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JoVE Journal Immunology and Infection
Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
 

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

Article DOI: 10.3791/57298-v • 11:35 min • May 4th, 2018
May 4th, 2018 •
*1, *1, 2, 1, 1, 2,3, 1,4, 1,4
1Department of Pediatrics, Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, 2Department of Molecular Biology, Massachusetts General Hospital, 3Department of Genetics, Harvard Medical School, 4Department of Pediatrics, Harvard Medical School
* These authors contributed equally

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Pseudomonas aeruginosa infection causes significant morbidity in vulnerable hosts. The nonredundant transposon insertion mutant library of P. aeruginosa strain PA14, designated as PA14NR Set, facilitates analysis of gene functionality in numerous processes. Presented here is a protocol to generate high-quality copies of the PA14NR Set mutant library.

Tags

Replication Ordered Nonredundant Library Pseudomonas Aeruginosa Strain PA14 Transposon Insertion Mutants High Quality Copies PA14NR Set Integrity Preservation Detailed Description Library Replication Quality Control Storage Bacterial Libraries Risk Of Contamination Step-by-step Instructions Global Scientific Community End User Visual Demonstration Cross-contamination Prevention Lab Coat Gloves Mask
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