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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients
 

Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients

Article DOI: 10.3791/61631-v • 07:25 min • July 16th, 2020
July 16th, 2020 •
1, 2, 1, 1, 1,3, 1,4
1Translational Medicine, Hospital for Sick Children, 2Center for Microbial Pathogenesis, Department of Pediatrics, University of Pittsburgh, 3Microbiology, Department of Pediatric Laboratory Medicine, Hospital for Sick Children, 4Infectious Diseases, Department of Pediatrics, Hospital for Sick Children

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This protocol provides methods for visualization of bacterial cells and polysaccharide synthesis locus (Psl) polysaccharide within the sputum of cystic fibrosis patients.

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Immunology and Infection Chronic Infection Lung Function Morbidity Antibiotic Therapy Pediatric Patients Spatial Organization Aggregation Biofilm Formation Polysaccharide Production Sputum Samples Tissue Clearing Technique Hydrogel Matrix 3D Structures Fluorescent Labels Fluorescence In Situ Hybridization Anti-Psl-antibodies Exopolysaccharide DAPI Staining Host Cells High-resolution Imaging
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