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复制的有序, 非冗余图书馆的铜绿假单胞菌应变 PA14 座子插入突变体
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
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复制的有序, 非冗余图书馆的铜绿假单胞菌应变 PA14 座子插入突变体

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11:35 min

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May 04, 2018

DOI:

10.3791/57298-v

DOI:
10.3791/57298-v

•

11:35 min
May 04, 2018

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1Department of Pediatrics, Mucosal Immunology and Biology Research Center,Massachusetts General Hospital, 2Department of Molecular Biology,Massachusetts General Hospital, 3Department of Genetics,Harvard Medical School, 4Department of Pediatrics,Harvard Medical School

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Pseudomonas AeruginosaPA14NR SetTransposon Mutant LibraryReplicationQuality ControlContamination PreventionSterile TechniquesReplicator Pin SterilizationLB Agar PlatesFreezer Storage

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Drenkard, E., Hibbler, R. M., Gutu, D. A., Eaton, A. D., Silverio, A. L., Ausubel, F. M., Hurley, B. P., Yonker, L. M. Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants. J. Vis. Exp. (135), e57298, doi:10.3791/57298 (2018).

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