Journal
/
Imunologia e Infecção
/
Replicación de la ordena, no redundante biblioteca de Pseudomonas aeruginosa cepa PA14 mutantes de inserción de transposones
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
Please note that all translations are automatically generated. Click here for the English version.

Replicación de la ordena, no redundante biblioteca de Pseudomonas aeruginosa cepa PA14 mutantes de inserción de transposones

7,905 Views

•

11:35 min

•

May 04, 2018

DOI:

10.3791/57298-v

DOI:
10.3791/57298-v

•

11:35 min
May 04, 2018

•

2 Views
, , , , , , ,
1Department of Pediatrics, Mucosal Immunology and Biology Research Center,Massachusetts General Hospital, 2Department of Molecular Biology,Massachusetts General Hospital, 3Department of Genetics,Harvard Medical School, 4Department of Pediatrics,Harvard Medical School

Tags

Pseudomonas AeruginosaPA14NR SetTransposon Mutant LibraryReplicationQuality ControlContamination PreventionSterile TechniquesReplicator Pin SterilizationLB Agar PlatesFreezer Storage

Read Article

Cite this Article

Drenkard, E., Hibbler, R. M., Gutu, D. A., Eaton, A. D., Silverio, A. L., Ausubel, F. M., Hurley, B. P., Yonker, L. M. Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants. J. Vis. Exp. (135), e57298, doi:10.3791/57298 (2018).

Download .ris file

Copy

Share Video

.

Copy