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High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection
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High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection
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11:50 min
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December 18, 2017
DOI:
1Veterans Affairs Greater Los Angeles Healthcare System, 2Departments of Medicine,David Geffen School of Medicine at University of California Los Angeles, 3Departments of Urology,David Geffen School of Medicine at University of California Los Angeles, 4Departments of Microbiology, Immunology, and Molecular Genetics,University of California Los Angeles
Summary
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We describe here a technique that combines transposon mutagenesis with high-throughput sequencing to identify and quantify transposon leptospiral mutants in tissues after a challenge of hamsters. This protocol can be used to screen mutants for survival and dissemination in animals and can also be applied to in vitro studies.
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Lourdault, K., Matsunaga, J., Evangelista, K. V., Haake, D. A. High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection. J. Vis. Exp. (130), e56442, doi:10.3791/56442 (2017).
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