Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture

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JoVE 신문
생물학

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JoVE 신문 생물학

Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture

DOI:

10.3791/675-v

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17:42 min

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February 13, 2008

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Georgia Woods, Karen Zito

¹Center for Neuroscience,University of California, Davis

Chapters

00:02Title
01:02Introduction
01:51Preparing DNA-Coated Gold Beads
05:36Coating Plastic Tubing
08:51Cutting the Plastic Tubing
10:18Shooting Tissue Slices
14:20Results
15:53Troubleshooting
17:04Conclusion

Summary

자동 번역

We describe a method for preparing DNA coated gold bullets and demonstrate the use of such bullets to biolistically transfect neurons in cultured hippocampal slices.

Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture

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Chapters

Summary

자동 번역

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