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Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture
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生物学
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JoVE Journal 生物学
Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture

Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture

DOI:
10.3791/675-v

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17:42 min

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February 13, 2008

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,
1Center for Neuroscience,University of California, Davis

章

  • 00:02標題
  • 01:02Introduction
  • 01:51Preparing DNA-Coated Gold Beads
  • 05:36Coating Plastic Tubing
  • 08:51Cutting the Plastic Tubing
  • 10:18Shooting Tissue Slices
  • 14:20結果
  • 15:53Troubleshooting
  • 17:04Conclusion

概要

自動翻訳

自動翻訳

We describe a method for preparing DNA coated gold bullets and demonstrate the use of such bullets to biolistically transfect neurons in cultured hippocampal slices.

タグ

Gene Gun BulletsBiolistic TransfectionNeuronsSlice CultureDNA Coated ParticlesRat Hippocampal SlicesGene Gun TransfectionFluorescent LabelingGold ParticlesPlastic TubingPlastic CartridgesBio-Rad Helios Gene Gun

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