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Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture
JoVE Journal
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JoVE Journal Biyoloji
Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture

Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture

DOI:
10.3791/675-v

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17:42 min

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February 13, 2008

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,
1Center for Neuroscience,University of California, Davis

Bölümler

  • 00:02Başlık
  • 01:02Introduction
  • 01:51Preparing DNA-Coated Gold Beads
  • 05:36Coating Plastic Tubing
  • 08:51Cutting the Plastic Tubing
  • 10:18Shooting Tissue Slices
  • 14:20Sonuçlar
  • 15:53Troubleshooting
  • 17:04Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We describe a method for preparing DNA coated gold bullets and demonstrate the use of such bullets to biolistically transfect neurons in cultured hippocampal slices.

Etiketler

Gene Gun BulletsBiolistic TransfectionNeuronsSlice CultureDNA Coated ParticlesRat Hippocampal SlicesGene Gun TransfectionFluorescent LabelingGold ParticlesPlastic TubingPlastic CartridgesBio-Rad Helios Gene Gun

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