Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture

Journal

Biologie

JoVE Journal
Biologie

This content is Free Access.

JoVE Journal Biologie

Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture

DOI:

10.3791/675-v

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17:42 min

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February 13, 2008

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Georgia Woods, Karen Zito

¹Center for Neuroscience,University of California, Davis

Kapitel

00:02Titel
01:02Introduction
01:51Preparing DNA-Coated Gold Beads
05:36Coating Plastic Tubing
08:51Cutting the Plastic Tubing
10:18Shooting Tissue Slices
14:20Ergebnisse
15:53Troubleshooting
17:04Conclusion

Summary

Automatische Übersetzung

We describe a method for preparing DNA coated gold bullets and demonstrate the use of such bullets to biolistically transfect neurons in cultured hippocampal slices.

Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture

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•

•

Kapitel

Summary

Automatische Übersetzung

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