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Journal / Immunology and Infection / マイコバクテリアの天然および組換え細胞外小胞の濃縮…
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Immunology and Infection

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JoVE Journal Immunology and Infection
マイコバクテリアの天然および組換え細胞外小胞の濃縮
 
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マイコバクテリアの天然および組換え細胞外小胞の濃縮

Article DOI: 10.3791/65138-v • 06:38 min • December 8th, 2023
December 8th, 2023 •
1, 1, 1,2, 1,3, 1, 1, 1, 1,4, 1, 1
1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 2Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute, Rutgers University

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このプロトコルでは、マイコバクテリウム・スメグマティス(Msm)の軸種培養からの天然マイコバクテリア細胞外小胞(mEV)の濃縮と、mCherry(赤色蛍光レポーター)含有組換えMsmEVsの設計と濃縮方法について詳しく説明します。最後に、結核菌のEsxAタンパク質を含むMsmEVを濃縮することで、新しいアプローチを検証します。

Tags

免疫学と感染症、第202号、細胞外小胞、組換え小胞、Esat-6、マイコバクテリア、ExsA、mCherry、Cfp-29、細菌
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