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Immunology and Infection

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JoVE Journal Immunology and Infection
Enriquecimiento de vesículas extracelulares nativas y recombinantes de micobacterias
 
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Enriquecimiento de vesículas extracelulares nativas y recombinantes de micobacterias

Article DOI: 10.3791/65138-v • 06:38 min • December 8th, 2023
December 8th, 2023 •
1, 1, 1,2, 1,3, 1, 1, 1, 1,4, 1, 1
1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 2Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute, Rutgers University

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Este protocolo detalla el enriquecimiento de vesículas extracelulares micobacterianas nativas (mEVs) de cultivos axénicos de Mycobacterium smegmatis (Msm) y cómo se pueden diseñar y enriquecer msmEVs recombinantes que contienen mCherry (un reportero fluorescente rojo). Por último, se verifica el novedoso enfoque con el enriquecimiento de MsmEVs que contienen la proteína EsxA de Mycobacterium tuberculosis.

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Immunology and Infection Número 202 Vesículas extracelulares vesículas recombinantes Esat-6 micobacterias ExsA mCherry Cfp-29 bacterias
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