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Journal / Immunology and Infection / 富集分枝杆菌的天然和重组细胞外囊泡…
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Immunology and Infection

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JoVE Journal Immunology and Infection
富集分枝杆菌的天然和重组细胞外囊泡
 
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富集分枝杆菌的天然和重组细胞外囊泡

Article DOI: 10.3791/65138-v • 06:38 min • December 8th, 2023
December 8th, 2023 •
1, 1, 1,2, 1,3, 1, 1, 1, 1,4, 1, 1
1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 2Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute, Rutgers University

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该方案详细介绍了从耻垢分枝杆菌（Msm）的无轴培养物中富集天然分枝杆菌细胞外囊泡（mEV），以及如何设计和富集含有mCherry（红色荧光报告基因）的重组MsmEV。最后，通过富集含有结核分枝杆菌EsxA蛋白的MsmEVs验证了新方法。

Tags

免疫学与感染，第 202 期，细胞外囊泡、重组囊泡、Esat-6、分枝杆菌、ExsA、mCherry、Cfp-29、细菌
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