Journal
/
생화학
/
An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model
JoVE 신문
생화학
Author Produced
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

2,776 Views

•

11:04 min

•

January 13, 2023

DOI:

10.3791/64391-v

DOI:
10.3791/64391-v

•

11:04 min
January 13, 2023

•

12 Views
, , , , , , , , , , ,
1Graduate Program in Pharmaceutical Sciences, Laboratory of Cancer Drug Resistance,Federal University of Parana, 2Graduate Program in Pharmaceutical Sciences,Federal University of Parana, 3Federal Institute of Parana

Summary

Automatically generated

Here, we describe a novel, simple, and low-cost device to successfully perform in vitro photodynamic therapy (PDT) assays using two-dimensional HeLa cell culture and verteporfin as a photosensitizer.

Explore More Videos

Photodynamic TherapyPDTLight-emitting DiodeLEDVerteporfinCytotoxicity2D Cell CultureIn-house-built DevicePhotoActLow-costHeLa CellsDulbecco’s Modified Eagle MediumCell Culture

Read Article

Cite this Article

Zanzarini, I. d. S., Barbosa, G., Prado, L. d. O., Zattoni, I. F., Da Paz, G., Prado, A. L. d., Volanski, W., Lavarda, M. D., Rego, F. G. d. M., Picheth, G., Moure, V. R., Valdameri, G. An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model. J. Vis. Exp. (191), e64391, doi:10.3791/64391 (2023).

Download .ris file

Copy

Share Video

.

Copy