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An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model
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生化学
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JoVE Journal 生化学
An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

2,776 Views

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11:04 min

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January 13, 2023

DOI:

10.3791/64391-v

DOI:
10.3791/64391-v

•

11:04 min
January 13, 2023

•

12 Views
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1Graduate Program in Pharmaceutical Sciences, Laboratory of Cancer Drug Resistance,Federal University of Parana, 2Graduate Program in Pharmaceutical Sciences,Federal University of Parana, 3Federal Institute of Parana

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Here, we describe a novel, simple, and low-cost device to successfully perform in vitro photodynamic therapy (PDT) assays using two-dimensional HeLa cell culture and verteporfin as a photosensitizer.

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Photodynamic TherapyPDTLight-emitting DiodeLEDVerteporfinCytotoxicity2D Cell CultureIn-house-built DevicePhotoActLow-costHeLa CellsDulbecco’s Modified Eagle MediumCell Culture

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Cite this Article

Zanzarini, I. d. S., Barbosa, G., Prado, L. d. O., Zattoni, I. F., Da Paz, G., Prado, A. L. d., Volanski, W., Lavarda, M. D., Rego, F. G. d. M., Picheth, G., Moure, V. R., Valdameri, G. An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model. J. Vis. Exp. (191), e64391, doi:10.3791/64391 (2023).

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