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An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model
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Biochemistry
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JoVE Journal Biochemistry
An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

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2,776 Views

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11:04 min

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January 13, 2023

DOI:

10.3791/64391-v

DOI:
10.3791/64391-v

•

11:04 min
January 13, 2023

•

12 Views
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1Graduate Program in Pharmaceutical Sciences, Laboratory of Cancer Drug Resistance,Federal University of Parana, 2Graduate Program in Pharmaceutical Sciences,Federal University of Parana, 3Federal Institute of Parana

Summary

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Here, we describe a novel, simple, and low-cost device to successfully perform in vitro photodynamic therapy (PDT) assays using two-dimensional HeLa cell culture and verteporfin as a photosensitizer.

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Photodynamic TherapyPDTLight-emitting DiodeLEDVerteporfinCytotoxicity2D Cell CultureIn-house-built DevicePhotoActLow-costHeLa CellsDulbecco’s Modified Eagle MediumCell Culture

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Zanzarini, I. d. S., Barbosa, G., Prado, L. d. O., Zattoni, I. F., Da Paz, G., Prado, A. L. d., Volanski, W., Lavarda, M. D., Rego, F. G. d. M., Picheth, G., Moure, V. R., Valdameri, G. An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model. J. Vis. Exp. (191), e64391, doi:10.3791/64391 (2023).

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