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JoVE Journal Biochemistry
An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model
 

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

Article DOI: 10.3791/64391-v • 11:04 min • January 13th, 2023
January 13th, 2023 •
1, 1, 1, 1, 1, 2,3, 2, 3, 2, 2, 1, 1
1Graduate Program in Pharmaceutical Sciences, Laboratory of Cancer Drug Resistance, Federal University of Parana, 2Graduate Program in Pharmaceutical Sciences, Federal University of Parana, 3Federal Institute of Parana

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Here, we describe a novel, simple, and low-cost device to successfully perform in vitro photodynamic therapy (PDT) assays using two-dimensional HeLa cell culture and verteporfin as a photosensitizer.

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Keywords: Photodynamic Therapy PDT Light-emitting Diode LED Verteporfin Cytotoxicity 2D Cell Culture In-house-built Device PhotoAct Low-cost HeLa Cells Dulbecco's Modified Eagle Medium Cell Culture
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