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Journal / Neuroscience / 一种从介观尺度到微观尺度的神经元成像组织清除方法…
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JoVE Journal Neuroscience
一种从介观尺度到微观尺度的神经元成像组织清除方法
 
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一种从介观尺度到微观尺度的神经元成像组织清除方法

Article DOI: 10.3791/63941-v • 07:20 min • May 10th, 2022
May 10th, 2022 •
1,2, 1,2,3, 1,2,4,5, 2,3, 6, 1,2,7
1Department of Neuroanatomy, Juntendo University Graduate School of Medicine, 2Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, 3Advanced Research Institute for Health Sciences, Juntendo University, 4Department of Neuroscience, Graduate School of Medicine, Kyoto University, 5Japan Society for the Promotion of Science, 6Department of Oral Anatomy and Neurobiology, Graduate School of Dentistry, Osaka University, 7Department of Multi-Scale Brain Structure Imaging, Juntendo University Graduate School of Medicine

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该协议提供了使用组织清除方法ScaleSF在脑切片中进行神经元成像的详细方法。该方案包括脑组织制备，组织澄清，清除切片的处理以及从介观到微观水平的神经元结构的共聚焦激光扫描显微镜成像。

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神经科学，第183期，
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