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Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture
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Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture

Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture

2,754 Views

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07:11 min

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August 25, 2022

DOI:

10.3791/63745-v

DOI:
10.3791/63745-v

•

07:11 min
August 25, 2022

•

9 Views
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1Department of Neurological Surgery,Cleveland Clinic, 2Cardiovascular and Metabolic Sciences,Lerner Research Institute, Cleveland Clinic

Summary

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Here, we describe a method of generating glioblastoma (GBM) organoids from primary patient specimens or patient-derived cell cultures and maintaining them to maturity. These GBM organoids contain phenotypically diverse cell populations and recreate tumor microenvironments ex vivo.

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GlioblastomaOrganoid CultureCellular DiversityMicroenvironmentDrug EfficacyResistancePersonalized MedicineTemperature-sensitive MaterialsSingle-cell SuspensionCell DetachmentNeurobasal Medium

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Cite this Article

Sundar, S. J., Shakya, S., Recinos, V., Hubert, C. G. Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture. J. Vis. Exp. (186), e63745, doi:10.3791/63745 (2022).

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