Here, we describe a method of generating glioblastoma (GBM) organoids from primary patient specimens or patient-derived cell cultures and maintaining them to maturity. These GBM organoids contain phenotypically diverse cell populations and recreate tumor microenvironments ex vivo.
Sundar, S. J., Shakya, S., Recinos, V., Hubert, C. G. Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture. J. Vis. Exp. (186), e63745, doi:10.3791/63745 (2022).