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Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture
JoVE Journal
Ricerca sul cancro
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JoVE Journal Ricerca sul cancro
Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture

Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture

DOI:
10.3791/63745-v

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07:11 min

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August 25, 2022

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, , ,
1Department of Neurological Surgery,Cleveland Clinic, 2Cardiovascular and Metabolic Sciences,Lerner Research Institute, Cleveland Clinic

Capitoli

  • 00:04Introduction
  • 01:25Making Organoid Molds
  • 02:22Preparing Single‐Cell Suspension from Glioblastoma (GBM) Adherent Culture
  • 03:22Making Organoids from a Single‐Cell Suspension
  • 05:23Results: Generation and Maintenance of Human GBM Diversity Ex Vivo from Patient‐Derived Cell Cultures
  • 06:10Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we describe a method of generating glioblastoma (GBM) organoids from primary patient specimens or patient-derived cell cultures and maintaining them to maturity. These GBM organoids contain phenotypically diverse cell populations and recreate tumor microenvironments ex vivo.

Tags

Keywords: GlioblastomaOrganoid CultureCellular DiversityMicroenvironmentDrug EfficacyResistancePersonalized MedicineTemperature-sensitive MaterialsSingle-cell SuspensionCell DetachmentNeurobasal Medium

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