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Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture
 

Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture

Article DOI: 10.3791/63745-v • 07:11 min • August 25th, 2022
August 25th, 2022 •
1, 2, 1, 2
1Department of Neurological Surgery, Cleveland Clinic, 2Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic

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Here, we describe a method of generating glioblastoma (GBM) organoids from primary patient specimens or patient-derived cell cultures and maintaining them to maturity. These GBM organoids contain phenotypically diverse cell populations and recreate tumor microenvironments ex vivo.

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Keywords: Glioblastoma Organoid Culture Cellular Diversity Microenvironment Drug Efficacy Resistance Personalized Medicine Temperature-sensitive Materials Single-cell Suspension Cell Detachment Neurobasal Medium
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