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Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation
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Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation

Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation

1,972 Views

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08:08 min

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January 12, 2022

DOI:

10.3791/63183-v

DOI:
10.3791/63183-v

•

08:08 min
January 12, 2022

•

5 Views
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1Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas,Universidad Nacional Autónoma de México, 2Departamento de Anatomía y Biología Celular and IDIVAL, Facultad de Medicina,Universidad de Cantabria

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Recombinant limbs are a powerful experimental model that allows for studying the process of cell differentiation and the generation of patterns under the influence of embryonic signals. This protocol presents a detailed method for generating recombinant limbs with chicken limb-mesodermal cells, adaptable to other cell types obtained from different organisms.

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Cite this Article

Marín-Llera, J. C., Fernández-Calderón, M., Chimal-Monroy, J. Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation. J. Vis. Exp. (179), e63183, doi:10.3791/63183 (2022).

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