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Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation
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JoVE Journal Gelişim Biyolojisi
Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation

Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation

1,971 Views

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08:08 min

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January 12, 2022

DOI:

10.3791/63183-v

DOI:
10.3791/63183-v

•

08:08 min
January 12, 2022

•

5 Views
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1Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas,Universidad Nacional Autónoma de México, 2Departamento de Anatomía y Biología Celular and IDIVAL, Facultad de Medicina,Universidad de Cantabria

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Recombinant limbs are a powerful experimental model that allows for studying the process of cell differentiation and the generation of patterns under the influence of embryonic signals. This protocol presents a detailed method for generating recombinant limbs with chicken limb-mesodermal cells, adaptable to other cell types obtained from different organisms.

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Marín-Llera, J. C., Fernández-Calderón, M., Chimal-Monroy, J. Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation. J. Vis. Exp. (179), e63183, doi:10.3791/63183 (2022).

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