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Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation
Chapters
- 00:04Introduction
- 00:38Embryo Incubation and Determination of Viability
- 01:06Obtaining Limb Mesodermal Cells to Fill Ectoderms
- 04:11Obtaining the Limb Ectoderms
- 05:21Assembling Mesodermal Cells Inside the Ectodermal Hull
- 06:04Transplantation of the Filled Ectoderm into a Host Embryo
- 06:51Results: Morphology and Patterning of Recombinant Limbs
- 07:40Conclusion
Recombinant limbs are a powerful experimental model that allows for studying the process of cell differentiation and the generation of patterns under the influence of embryonic signals. This protocol presents a detailed method for generating recombinant limbs with chicken limb-mesodermal cells, adaptable to other cell types obtained from different organisms.
