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Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation

Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation

DOI:
10.3791/63183-v

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08:08 min

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January 12, 2022

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, ,
1Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas,Universidad Nacional Autónoma de México, 2Departamento de Anatomía y Biología Celular and IDIVAL, Facultad de Medicina,Universidad de Cantabria

Capitoli

  • 00:04Introduction
  • 00:38Embryo Incubation and Determination of Viability
  • 01:06Obtaining Limb Mesodermal Cells to Fill Ectoderms
  • 04:11Obtaining the Limb Ectoderms
  • 05:21Assembling Mesodermal Cells Inside the Ectodermal Hull
  • 06:04Transplantation of the Filled Ectoderm into a Host Embryo
  • 06:51Results: Morphology and Patterning of Recombinant Limbs
  • 07:40Conclusion

Summary

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Recombinant limbs are a powerful experimental model that allows for studying the process of cell differentiation and the generation of patterns under the influence of embryonic signals. This protocol presents a detailed method for generating recombinant limbs with chicken limb-mesodermal cells, adaptable to other cell types obtained from different organisms.

Tags

Chicken Recombinant LimbsCell DifferentiationMorphogenesisPatterningEmbryonic SignalsIn VivoStem CellsProgenitor CellsTrypsinCollagenaseDMEMFBSCell Strainer

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