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Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity
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Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity

Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity

1,553 Views

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06:12 min

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February 10, 2022

DOI:

10.3791/63166-v

DOI:
10.3791/63166-v

•

06:12 min
February 10, 2022

•

6 Views
,
1Biomedical Research Institute,National Institute of Advanced Industrial Science and Technology, 2DBT-AIST International Laboratory for Advanced Biomedicine,National Institute of Advanced Industrial Science and Technology

Summary

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A mechanical brain injury model in the adult zebrafish is described to investigate the molecular mechanisms regulating their high regenerative capacity. The method explains to create a stab wound injury in the optic tectum of multiple species of small fish to evaluate the regenerative responses using fluorescent immunostaining.

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Stab Wound InjuryOptic TectumZebrafishMedakaRegenerative CapacityBrain InjuryRGC ProliferationDifferentiationNeedle-mediated InjuryCentral Nervous SystemRegenerative TherapyPBS PerfusionParaformaldehyde Fixation

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Cite this Article

Shimizu, Y., Kawasaki, T. Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity. J. Vis. Exp. (180), e63166, doi:10.3791/63166 (2022).

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