Journal
/
发育生物学
/
Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity

Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity

1,553 Views

•

06:12 min

•

February 10, 2022

DOI:

10.3791/63166-v

DOI:
10.3791/63166-v

•

06:12 min
February 10, 2022

•

6 Views
,
1Biomedical Research Institute,National Institute of Advanced Industrial Science and Technology, 2DBT-AIST International Laboratory for Advanced Biomedicine,National Institute of Advanced Industrial Science and Technology

Summary

Automatically generated

A mechanical brain injury model in the adult zebrafish is described to investigate the molecular mechanisms regulating their high regenerative capacity. The method explains to create a stab wound injury in the optic tectum of multiple species of small fish to evaluate the regenerative responses using fluorescent immunostaining.

Explore More Videos

Stab Wound InjuryOptic TectumZebrafishMedakaRegenerative CapacityBrain InjuryRGC ProliferationDifferentiationNeedle-mediated InjuryCentral Nervous SystemRegenerative TherapyPBS PerfusionParaformaldehyde Fixation

Read Article

Cite this Article

Shimizu, Y., Kawasaki, T. Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity. J. Vis. Exp. (180), e63166, doi:10.3791/63166 (2022).

Download .ris file

Copy

Share Video

.

Copy