Journal
/
Developmental Biology
/
Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity

Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity

English

Automatically Generated

1,545 Views

•

06:12 min

•

February 10, 2022

DOI:

10.3791/63166-v

DOI:
10.3791/63166-v

•

06:12 min
February 10, 2022

•

6 Views
,
1Biomedical Research Institute,National Institute of Advanced Industrial Science and Technology, 2DBT-AIST International Laboratory for Advanced Biomedicine,National Institute of Advanced Industrial Science and Technology

Summary

Automatically generated

A mechanical brain injury model in the adult zebrafish is described to investigate the molecular mechanisms regulating their high regenerative capacity. The method explains to create a stab wound injury in the optic tectum of multiple species of small fish to evaluate the regenerative responses using fluorescent immunostaining.

Explore More Videos

Stab Wound InjuryOptic TectumZebrafishMedakaRegenerative CapacityBrain InjuryRGC ProliferationDifferentiationNeedle-mediated InjuryCentral Nervous SystemRegenerative TherapyPBS PerfusionParaformaldehyde Fixation

Read Article

Cite this Article

Shimizu, Y., Kawasaki, T. Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity. J. Vis. Exp. (180), e63166, doi:10.3791/63166 (2022).

Download .ris file

Copy

Share Video

.

Copy