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Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity
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Capítulos
- 00:04Introduction
- 00:55Stab Wound Injury in the Adult Optic Tectum
- 01:34Brain Dissection
- 03:01Preparation of Frozen Sections
- 04:44Results: Fluorescent Immunostaining Against RGC Proliferation and the Generation of Newborn Neurons After Tectum Injury
- 05:28Conclusion
A mechanical brain injury model in the adult zebrafish is described to investigate the molecular mechanisms regulating their high regenerative capacity. The method explains to create a stab wound injury in the optic tectum of multiple species of small fish to evaluate the regenerative responses using fluorescent immunostaining.
