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Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies
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JoVE Journal Biyokimya
Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

DOI:
10.3791/63015-v

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04:22 min

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November 20, 2021

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, , , ,
1School of Molecular Sciences,Arizona State University, 2Center for Applied Structural Discovery, Biodesign Institute,Arizona State University, 3Eyring Materials Center,Arizona State University, 4Department of Microbiology and Immunology,The Pennsylvania State University

Özet

Otomatik Çeviri

Otomatik Çeviri

Lipid monolayers have been used as a foundation for forming two-dimensional (2D) protein crystals for structural studies for decades. They are stable at the air-water interface and can serve as a thin supporting material for electron imaging. Here we present the proven steps on preparing lipid monolayers for biological studies.

Etiketler

Lipid MonolayerElectron Crystallography2D CrystallizationMembrane ProteinsProtein BindingSample PreparationTeflon PlateBuffer ReservoirHumidity ControlCarbon-coated EM Grid

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