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Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons
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Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons

Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons

2,031 Views

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11:52 min

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August 26, 2021

DOI:

10.3791/62726-v

DOI:
10.3791/62726-v

•

11:52 min
August 26, 2021

•

9 Views
, , , , ,
1Department of Neurology,Johns Hopkins University

Summary

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We describe a method for differentiating spinal cord human induced pluripotent-derived astrocytes and neurons and their co-culture for electrophysiological recording.

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Human IPSCsAstrocytesNeuronsSpinal CordAmyotrophic Lateral Sclerosis (ALS)Neural Progenitor CellsDifferentiationElectrophysiologyCell CultureDissociationMedium ExchangeTrypsinPBSPipetteCell AggregatesCell Monolayer37°C Incubation

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Cite this Article

Taga, A., Habela, C. W., Johns, A., Liu, S., O'Brien, M., Maragakis, N. J. Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons. J. Vis. Exp. (174), e62726, doi:10.3791/62726 (2021).

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