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Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons
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JoVE 杂志 神经科学
Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons

Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons

DOI:
10.3791/62726-v

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11:52 min

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August 26, 2021

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, , , , ,
1Department of Neurology,Johns Hopkins University

Chapters

  • 00:04Introduction
  • 00:48Maintaining Non-Confluent Human Induced Pluripotent Stem Cells
  • 02:58Diffrentiation of NPC into Motor Neuron
  • 03:46Co-Culturing Motor Neurons and Astrocytes in Multi-Electrode Array Plates
  • 07:42Multi-Electrode Array Recording
  • 08:16Pharmacological Assays on Multi-Electrode Array
  • 09:01Results: Human Pluripotent Stem Cell-Derived Motor Neurons and Astrocytes
  • 11:09Conclusion

Summary

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We describe a method for differentiating spinal cord human induced pluripotent-derived astrocytes and neurons and their co-culture for electrophysiological recording.

Tags

Human IPSCsAstrocytesNeuronsSpinal CordAmyotrophic Lateral Sclerosis (ALS)Neural Progenitor CellsDifferentiationElectrophysiologyCell CultureDissociationMedium ExchangeTrypsinPBSPipetteCell AggregatesCell Monolayer37°C Incubation

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