Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons

Arens Taga; Christa W. Habela; Alexandra Johns; Shiyu Liu; Mollie O'Brien; Nicholas J. Maragakis

doi:10.3791/62726

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Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons

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神経科学

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JoVE Journal 神経科学

Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons

2,031 Views

•

11:52 min

•

August 26, 2021

DOI:

10.3791/62726-v

DOI:

10.3791/62726-v

•

11:52 min

August 26, 2021

•

9 Views

Arens Taga*¹, Christa W. Habela*¹, Alexandra Johns¹, Shiyu Liu¹, Mollie O’Brien¹, Nicholas J. Maragakis¹

¹Department of Neurology,Johns Hopkins University

概要

We describe a method for differentiating spinal cord human induced pluripotent-derived astrocytes and neurons and their co-culture for electrophysiological recording.

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Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons

概要

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