Dergi
/
Nörobilim
/
Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons

Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons

DOI:
10.3791/62726-v

•

11:52 min

•

August 26, 2021

•

, , , , ,
1Department of Neurology,Johns Hopkins Üniversitesi

Bölümler

  • 00:04Introduction
  • 00:48Maintaining Non-Confluent Human Induced Pluripotent Stem Cells
  • 02:58Diffrentiation of NPC into Motor Neuron
  • 03:46Co-Culturing Motor Neurons and Astrocytes in Multi-Electrode Array Plates
  • 07:42Multi-Electrode Array Recording
  • 08:16Pharmacological Assays on Multi-Electrode Array
  • 09:01Results: Human Pluripotent Stem Cell-Derived Motor Neurons and Astrocytes
  • 11:09Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We describe a method for differentiating spinal cord human induced pluripotent-derived astrocytes and neurons and their co-culture for electrophysiological recording.

Etiketler

Human IPSCsAstrocytesNeuronsSpinal CordAmyotrophic Lateral Sclerosis (ALS)Neural Progenitor CellsDifferentiationElektrofizyolojiCell CultureDissociationMedium ExchangeTrypsinPBSPipetteCell AggregatesCell Monolayer37°C Incubation

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids

Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format

Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips

Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation

Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors

Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons

Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia

Read Article