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Analyse der neutralen Lipidsynthese in Saccharomyces cerevisiae durch metabolische Markierung und Dünnschichtchromatographie
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Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography
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Analyse der neutralen Lipidsynthese in Saccharomyces cerevisiae durch metabolische Markierung und Dünnschichtchromatographie

2,869 Views

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08:30 min

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February 02, 2021

DOI:

10.3791/62201-v

DOI:
10.3791/62201-v

•

08:30 min
February 02, 2021

•

1 Views
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1Department of Cell Biology,University of Texas Southwestern Medical Center

Summary

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Hier wird ein Protokoll zur metabolischen Markierung von Hefe mit 14C-Essigsäure vorgestellt, das mit dünnschichtiger Chromatographie zur Abtrennung neutraler Lipide gekoppelt ist.

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Metabolic LabelingThin Layer ChromatographyLipid MetabolismSaccharomyces CerevisiaeYeast CultureRadiolabelingQuenching BufferCentrifugationLipid SynthesisEnzyme Regulation

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Cite this Article

Rogers, S., Henne, W. M. Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography. J. Vis. Exp. (168), e62201, doi:10.3791/62201 (2021).

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